Water contributes to the synthesis and ultrafast synthesis of collagen-like synthetic poly-L-proline.

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In the process published online now at National Science Review, an organization led by Prof. Hua Lu from the College of Chemistry and Molecular Engineering at Peking University shared some surprising findings. In the process of studying ProNCA’s ROP, a type of polymerization performed in a dry container, the team discovered ultrafast polymerization and controlled liquids.

The ProNCA monomer can be melted and the solubility of the PLP product in organic solvents is very low. Because of this problem, the ROP of ProNCA lasted longer than one week in dry organic solvents, providing oligomers with less power in molecular weight and dispersion. The new method solves the problem by simply replacing the anhydrous organic solvent with a mixed aqueous-acetonitrile solvent, and the polymerization process can be completed in 30 seconds to obtain an oligoproline with a degree of polymerization (DP) of 20, or PLP with a. DP up to 200 in 5 minutes.

Due to the very rapid rate of polymerization, more than most side effects such as monomer hydrolysis, the ROP is highly controlled, which is shown to be highly dependent on the compound, growth. Measurement of the chain kinetic pattern, predictable molecular weight, and narrow dispersity, and wide area of ​​the initiator. Mechanistic experiments and computational analysis (DFT) also show that water acts as a proton shuttle during polymerization, aiding the movement of the proton to the numerical determination stage. , then lower the final energy balance to as much as 7.1 kcal / mol and speed up the. extreme polymerization.

Due to its low molecular weight, easy operation, and easy cleaning, the researchers combined asparaginase-PLP conjugates with the use of asparaginase, an anticancer drug, as a starter. In collaboration with the team of Professor Song Yuqin from Peking University Hospital, the researchers found that the asparaginase-PLP conjugate can prolong the blood flow of asparaginase, improving antitumor efficacy. in vivo, and reduces immunogenicity compared with wild- type protein drugs. Both research teams are ready to re -conduct in -depth preclinical research, and to treat patients with acute lymphoblastic leukemia and NK / T cell lymphoma in the future. In addition, Prof. Lu believes PLP itself is also useful in the food industry, cosmetics, and other economic applications.







The living ROP of ProNCA. The monomers were dissolved in an aqueous-acetonitrile solvent, and when the researchers added the starting reaction, the polymerization reaction immediately started and released a large amount of carbon dioxide, and the reaction ended in of one minute. Published: Science China Press

Equal control over the process and dispersion of multiblock copolymers


More information:
Yali Hu et al, water-assisted and protein-fast initiation and binding of the polymerization ring of Proline N-Carboxyanhydride, National Science Review (2022). DOI: 10.1093 / nsr / nwac033

Presented by Science China Press

Directions: Water aids in the unpredictable and ultrafast synthesis of collagen-like synthetic poly-L-proline (2022, April 1) Retrieved 2 April 2022 from https://phys.org/news/2022 -04-unexpected-ultrafast-synthesis- collagen-like-synthetic.html

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