Scientists at Scripps Research have unveiled a new meat purification method for melting large organic samples. It is easy for science to observe and study the biological processes involved in diseases that exist among all body systems.
It is explained in a paper on Nature on March 28, 2022, dubbed HYBRiD, the new version incorporates the ingredients of the first two basic ingredients into meat cleansing technology, making it easier and more scalable than most applications. .
“This is a simple and universal process for researching large body parts or whole animals,” said lead author Li Ye, Ph.D., a professor of neuroscience at Scripps Research.
Cleansing the meat involves using solvents to remove molecules that melt the meat (such as fat), making the meat visually appealing – while keeping it fresh. most of the proteins and structures. Scientists typically use genetically engineered fluorescent beams or antibody-linked fluorescent beacons to signal strong genes or other molecules of interest in the lab animal, and the cleaning the flesh in such a way that these lights can be shot at the same time throughout the animal.
Scientists began developing natural cleansing techniques about 15 years ago, for the purpose of finding nerve connections throughout the brain. While these techniques work well for the brain, they do not work well when used on other body parts or the whole body, which is in buildings that are difficult to destroy.
These methods have so far used organic solvents or liquid solvents. The former is faster and more powerful but reduces fluorescent signals. The use of water-solvents is better at maintaining fluorescence but very weak for cleaning non-brain tissue. In addition, both methods require heavy -duty processes, often using hazardous chemicals.
“No standard lab can use these standard methods and in a big way,” said Yu Wang, a graduate student at Ye laboratory who was the paper’s first author.
The new method developed by Ye and his team uses a combination of natural solvents and detergents, and uses water -based hydrogels to prevent that. roots in the flesh need to be preserved. It is not often necessary to pump solvents through the sample.
“In many cases, you can put everything in a jar and keep it in a shaker at your seat until it’s done,” says former author Victoria. Nudell, a research fellow at the Ye lab. “This simplifies the process and can be scaled up for normal use.”
Researchers have demonstrated the simplicity and effectiveness of their new approach to applications. This was done in collaboration with the office of John Teijaro, Ph.D., associate professor of immunology and microbiology, to extract SARS-CoV-2-infected cells into whole mouse breasts for first time – a simple, modern procedure that can be performed in a high -rise biosafety room where access to equipment is limited.
He and his team are working with their scientific colleagues on many applications of the new technology, including the search for nerve pathways throughout the body.
“HYBRiD: hydrogel-reinforced DISCO for the cleansing of mammalian bodies” co-authored by early authors Victoria Nudell and Yu Wang, and by Zhengyuan Pang, Neeraj Lal, Min Huang, Namir Shaabani, Wesam Kanim, John Teijaro, Anton Maximov, and Li Ae all completed Scripps Research during the course of the study.
The new technology can be seen in the body and the whole body
Victoria Nudell et al, HYBRiD: hydrogel-reinforced DISCO for washing mammalian bodies, Nature (2022). DOI: 10.1038 / s41592-022-01427-0
Presented by The Scripps Research Institute
Directions: A new way to prepare meat for faster learning in many diseases (2022, March 29) Retrieved 29 March 2022 from https://phys.org/news/2022 -03-method-tissue-transparent-diseases.html
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