The discovery of a new phenomenon is a game changer for the efficient bioproduction of essential chemicals

The discovery of a new phenomenon is a game changer for the efficient bioproduction of essential chemicals

The G6P secret engine was introduced in this study. Available: Kobe University

A research team has discovered a new mechanism that secretes glucose captured by E. coli from cell bacteria such as glucose-6-phosphate (G6P). This research is important for the improvement of the methods that consistently produce good perfumes and raw materials for the medicine, food and chemical industries (among others) from the biomass. The research team included Associate Professor Tanaka Tsutomu of Kobe University’s Graduate School of Engineering, Special Postdoctoral Researcher Fujiwara Ryosuke and Noda Shuhei et al. of RIKEN Center for Sustainable Resource Science, and Professor Umetsu Mitsuo et al. of the University of Tohoku School of Engineering.

Based on the identified technology, the researchers have successfully developed a new technology to increase the performance of the captured compound by combining metabolic engineering and cell surface engineering.

It is hoped that by using this method, the amount of solvent can be significantly increased by adding a small amount of ‘metabolic acid’ to the microbe culture broth.

These research findings are published in Metabolic Engineering and March 5, 2022.

The big ones

  • The researchers found that part of the glucose captured by E. coli is secreted as glucose-6-phosphate (G6P).
  • They successfully developed a new technology that can increase the activity of different compounds by binding to the secreted G6P on the surface of bacteria.
  • It is thought that the new method could be used on ‘metabolic compounds’. This allows the production of a large number of beneficial compounds by adding a small fraction of the ‘metabolic acid’ to the microbe culture.

Further research

A major advantage of products derived from biomass (low costs that can be greatly modified by nature, such as grass and trees) is that they are carbon -free; They do not increase production and emit CO into the air2 pae. In bioproduction technologies, this biomass is used as a raw material and microbes use it to make fixed compounds (e.g., fragrances for the chemical, pharmaceutical and food industries, etc.). among others). The development of these technologies addresses the SDGs and is critical to achieving a low carbon society.

The discovery of a new phenomenon is a game changer for the efficient bioproduction of essential chemicals

The images show a significant increase in phenylalanine activity. Available: Kobe University

Professor Tanaka’s research team demonstrated biomass decomposition enzymes on the surface of various microbes, and developed cell surface engineering technology to improve the spatial resolution of biomass. Microbes reduce sugars found in plant biomass such as cellulose and cellooligosaccharide to glucose. In their first research, the researchers metabolically treated microbes that captured glucose that was degraded by the microbes and used only to make the target compound.

During this study, they discovered something new that had nothing to do with biomass degradation. They found that the expression of the enzyme increased the activity of the compound in question. Using this method, they show that it is possible to increase the size and volume of production of the biomass-derived compound, allowing for decarbonization of the material. The team then did some new research with the idea of ​​understanding the mechanism behind this event and using metabolic agents.

Type of research

This research team witnessed a recent phenomenon in which a portion of the glucose captured by the E. coli microorganism was released from the bacterial cell as glucose-6-phosphate (G6P). Normally, microbes constantly convert glucose (their food source) to G6P and capture it in their cells. To date, it is thought that G6P has not been expelled from captured bacterial cells. This study presented a new mechanism by which E. coli releases G6P, contrary to popular belief. The biomass degradation enzyme is expressed in the cell membrane of the microbe and releases G6P. The researchers found that short -term binding of this G6P stimulated metabolism in E. coli cells to increase the activity of the binding compound.

Next, the research team used this technique to isolate different proteins that can bind G6P on the surface of the bacterial cell. In this way, they developed a new technology to enhance the company’s founding. They have successfully demonstrated the new technology in the production of a large amount of the aromatic amino acid phenylalanine.

  • The discovery of a new phenomenon is a game changer for the efficient bioproduction of essential chemicals

    Application of new technology in tyrosine and muconic acid production. Available: Kobe University

  • The discovery of a new phenomenon is a game changer for the efficient bioproduction of essential chemicals

    Figure showing the ‘metabolic spice’ techniques: By adding a small portion of the ‘metabolic spice’ to the culture dough, the composition of the mixture can be greatly increased. Available: Kobe University

In addition, they increased the production of the aromatic amino acid tyrosine and muconic acid (an essential Dicarboxylic acid) by using the built -in technology. Muconic acid, meanwhile, is essential and essential to the profession. It can be easily converted to adipic acid, an additive in nylon production and is also used as a raw material in the manufacture of various pharmaceuticals and chemical products. These results show that this new technique can be used to make chemical compounds.


The research team confirmed that, in contrast to the expression of proteins on the surface of bacterial cells, the same phenomenon can be performed by adding a specific molecule to the broth culture. Researchers have called the small molecules that contain this type of ‘metabolic spice’ and are working on developing them. They hope to increase the composition of the compound by incorporating a small amount of odor into the microbe’s culture, without the need to genetically interact with the microbe.

In addition, this technology enhances specific metabolic pathways and increases the delivery of precursors to various nutrients). It can be used even if the final product cannot be determined. In addition, the results of this study showed that the low level of selection and association with G6P was sufficient.

The nature of the metabolic spice differs from the methods available for the detection of chemicals with high selectivity and relevance. Therefore, there may be new candidates for the metabolic pathway among the ‘rejected chemicals’ stored in chemical warehouses maintained by different industries. This is expected to lead to new academia-industry companies with industries not previously involved in bioproduction.

New metabolic technology improves the bioproduction of polymer products

More information:
Ryosuke Fujiwara et al, G6P capture molecules in the periplasm of Escherichia coli accelerate the shikimate pathway, Metabolic Engineering (2022). DOI: 10.1016 / j.ymben.2022.03.002

Presented by Kobe University

Directions: Discovery as a game changer for the efficient bioproduction of essential chemicals (2022, April 13) Retrieved 14 April 2022 from -game-changer-efficient- bioproduction.html

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