Retro technology promotes new bacterial technology for bioenergy

Hoʻonui ka ʻenehana Retro i ka ʻenehana bacteria hou no ka bioenergy

ORNL scientists have developed a new microbial mapping system that improves traditional protoplast fusion technologies to identify genes that promote desired genetic traits such as biomass improvement. Found: Nathan Armistead / ORNL, US Dept. of energy. Reprinted with permission of Oxford University Press, publisher of Nucleic Analysis

Scientists at Oak Ridge National Laboratory have struggled to document the genomes of bacteria to better understand the origin of their physical characteristics and improve their performance for bioenergy production. The method they used best for the labeling of biofuels for biofuels relied on the production and observation of various forms of re -engineering, although the bacterial infections that reproduce asexually with little difference.

“Bacteria no longer work the way we should. They often multiply in division, where you go from a mother cell to two daughter cells that have the same genome as the parent,” said Josh Michener. project leader in ORNL’s Biosciences Division.

The result came in a genetic research tool that was first developed in the 1970s: protoplast fusion. Scientists have been able to trace Bacillus traditions using this technology, and have produced a large number of genetically diverse organisms, which provide a wealth of insight into genomic mutation and the impact of physical characteristics.

Protoplast fusion is a common mechanical process in which cells are removed from their outer layer and combined with chemistry, to allow re -fusion between the chromosomes of the parents. Although researchers have used the technology for many years, the former as a natural genetic technique and as a tool to engineer traits such as antibiotic action on microorganisms, is unclear. in chromosomal reorganization.

“When scientists used this protoplast fusion technology in the past, they didn’t really know how the genomes of the progeny were due to the technological limitations of the sun,” Michener said. “We’ve been able to revitalize the technology and make it right. We’ve been blessed with 50 years of developing the school so we’ve been able to make this kind of cross and then look at hundreds to hundreds of children. “

The researchers used statistical methods to examine the genomes of the resulting eggs and transcribe them to their parents. “We can say, this little piece of DNA in the genome is from parent A and then a little piece from parent B, and so on,” Michener said.

They have reconnected into the genome in the form of long scales, as shown in Nucleic Analysis. “We’ve got small pieces and big pieces of DNA transformation, and the process is very simple.

The company later completed four rounds of reunification, with about 500 families registered and transferred. A numerical systems biology team led by Dan Jacobson of ORNL is using machine learning techniques to speed up phenotyping and mapping on bacterial panels to identify links between body characteristics. desired and their genetic basis.

The result is the ability to record new microbial species that can accelerate the growth of microbes, for example, making it more efficient to break down plant biomass for the production of biofuels. clean.

“One of the things that was brought out of the program was a lot of accurate knowledge of ancient literature,” Michener said. “Sometimes it’s not about the latest and snazziest way.”

The knowledge adds critical forces to the genetic map identified by the Center for Bioenergy Innovation at ORNL, Michener added. Other members of the research team were Delyana Vasileva, Jared Streich, Leah Burdick, Dawn Klingeman, Hari Chhetri, Christa Brelsford, Chris Ellis and Dan Close.


Identifying important recombinant plants for production


More information:
Delyana P Vasileva et al, Protoplast fusion of Bacillus species to produce frequent, inconsistent, genome-wide homologous recombination, Nucleic Analysis (2022). DOI: 10.1093 / nar / gkac025

Provided by Oak Ridge National Laboratory

Directions: Retro technology promotes new bacterial technology for bioenergy (2022, April 6) Retrieved 7 April 2022 from https://phys.org/news/2022-04-retro-technique-advances-modern- bacterial.html

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