It is also incorporated into the body to regenerate cartilage

Hoʻohui pū ʻia i loko o ke kino no ka hana hou ʻana o ka cartilage

a) A model of a 3D TGFβ-BMSC-IHI nanoscaffold. b) The composition of gelatin-coated and TGF-β3-loaded MnO2 NTs. c) The FESEM image showed that most of the BMSCs interact with other cells and 1D fibril-like structures, such as the structures of natural tissues. d) By remodeling the oxidative microenvironment, increasing cell life, and chondrogenesis of the altered tissues, cartilage regeneration can finally be achieved. Published: Science China Press

A study led by Prof. Qiuyu Zhang (Northwestern Polytechnical University), Prof. Ki-Bum Lee (Rutgers University), and Prof. Liang Kong (School of Stomatology, The Fourth Military Medical University) developed an injectable hybrid inorganic (IHI) nanoscaffold-templated stem cell assembly and used it to repair large cartilage defects.

Cartilage injuries are often plagued and most of them do not have treatment due to the low risk of cartilage tissue regeneration. The advent of 3D stem cell culture systems has led to advances in developmental biology, disease modeling, and modern medicine. For example, primary cells can be first modified to reduce inflammation in areas of cartilage injuries and then divide into cartilage cells (e.g., chondrocytes) to restore function.

However, there are important barriers that remain to be overcome before the curative potential of stem cell therapies can be realized. The limitation on the chondrogenic separation of cells in vivo has often resulted in regenerative changes. In addition, due to the spread of oxidative stress and inflammation in the microenvironment of the injured areas, stem cells often undergo apoptosis after injection.

To address these problems, the researchers reported on the development of a 3D IHI nanoscaffold-templated stem cell assembly system for advanced 3D stem cell culture and implantation. 3D-IHI nanoscaffold rapidly binds to cells in injectable tissues constructed by incorporating 3D cell-cell and cell-matrix interactions, delivering deep and homogeneously chondrogenic proteins into tissues. Integrated 3D culture system, and promote chondrogenesis through nanotopographical effects.

When implanted in vivo in a rabbit cartilage injury model, 3D-IHI nanoscaffold effectively alters the dynamic microenvironment after cartilage injury by combining the regenerative cues described in first, and at the same time scavenges reactive oxygen species using the manganese dioxide synthesis. In this way, rapid correction of cartilage deficiencies is seen with muscle regeneration and restoration of function in the short and long term. Given the excellent versatility and therapeutic effect of 3D-IHI nanoscaffold-based cartilage regeneration, it can provide promising avenues to advance a variety of specific technology applications.

The research is published in National Science Review.

  • It is also incorporated into the body to regenerate cartilage

    a) A schematic diagram showing the 3D-IHI nanoscaffold can enhance the chondrogenic differentiation of BMSC through synergy between N-cadherin and FAK-mediated pathways. b) Strong bonds between MnO2 NTs and conventional associations are well supported by ECM proteins in cellular synthesis as shown in the SEM image. c) Bicinchoninic acid assay showed increased absorption of gelatin from MnO2 nanotubes compared with control groups. d) In MnO2 The type of nanotube assembly modeled significantly enhanced the cell-matrix interaction as demonstrated by the modified expression patterns of the FAK gene. e) Representative immunostaining images showing improved chondrogenesis of BMSCs in BMSC-IHI nanoscaffold groups compared with control groups. Scale: 50 μm. fh) The expression of chondrogenic genes, including SOX9 (f), Aggrecan (g), and Col-II (h) was demonstrated by qRT-PCR analysis. Published: Science China Press

  • It is also incorporated into the body to regenerate cartilage

    a) Diagram showing the cutting process and the time of cartilage repair. The degradation of MnO2 NTs and the reconstruction procedure can be viewed through MRI. b) To identify our mutations, the BMSCs are labeled with a fluorescent green protein (GFP). Scale: 100 μm. c) The red fluorescent signals of the ROS probe were significantly reduced in MnO.2 NTs in the IHI nanoscaffold can effectively deplete ROS at the defect site. The proliferation of cell proliferation was confirmed by the high expression of the proliferative marker Ki67 immunostaining. Scale: 50 μm. d) The TGFβ-BMSC-IHI nanoscaffold can capture the number of cells after conversion compared with other cell transfer groups by counting the number of GFP + cells remaining in (c). e) The histogram of the fluorescence intensity of the ROS probe shows the effective use of ROS in MnO.2 NT found associations. f) The number of Ki67 + cells in defects. The numbers in (e) and (f) are based on the intensity of the fluorescence in (c). Published: Science China Press

  • It is also incorporated into the body to regenerate cartilage

    a) A photograph showing the regeneration process of cartilage over a long period of time (3 months). b) Cartilage regeneration was observed in vivo through H&E, Safranin O staining, Col-II immunochemistry staining, and macroscopic imaging. Increase in scale: 2 mm, increase in scale: 200 μm. ch) High cartilage thickness (by H&E staining) (c), cartilage (by Safranin O staining) (d), ECM material (by Col II immunostaining) (e). The results of the International Cartilage Repair Society (ICRS) macroscopic (f) and histologic scores (g) showed a significant improvement in defect repair techniques in the TGFβ-BMSC-IHI nanoscaffold group. The Osteoarthritis Research Society International (OARSI) has shown that the TGFβ-BMSC-IHI nanoscaffold can prevent the development of osteoarthritis (h). Published: Science China Press

The availability of the TRPV4 gene to regulate cartilage growth could lead to future therapies for joint repair.

More information:
Shenqiang Wang et al, Injectable hybrid inorganic nanoscaffold as a high -speed combination model for cartilage repair, National Science Review (2022). DOI: 10.1093 / nsr / nwac037

Presented by Science China Press

Directions: Injectable stem cell assembly for cartilage regeneration (2022, April 15) accessed 15 April 2022 from

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