Deep osmolyte has applications in molecular machinery

Loaʻa ka osmolyte hohonu i nā noi i nā mīkini molekala

Fluorescence microscopy images of kinesin-driven MTs show the effect of trimethylamine N-oxide (TMAO) on the conformation of microtubules (MTs). With the addition of the concept of TMAO, given below the figures, the MTs are simple; scale bar: 5 mm. aie: ACS Omega (2022). DOI: 10.1021 / acsomega.1c06699

Kinesin and microtubules (MTs) are the main components of the cytoskeleton in the cells of living things. Kinesin and microtubules play important roles in a wide range of cellular functions, especially intracellular transport. Advances in bioengineering and biotechnology make it possible to use these natural molecules as part of molecular machines and molecular robots. The in vitro gliding assay is the best way to evaluate the potential of these biomolecules for molecular mechanisms.

A group of scientists led by Prime Minister Arif Md. Rashedul Kabir of the University of Hokkaido demonstrated a simple and straightforward way to convert and strengthen the complexity of kinesin -induced MTs. Their information is published in ACS Omegaa journal published by the American Chemical Society (ACS).

In the in vitro gliding assay, kinesin molecules are attached to a base material, and accelerate into MTs as molecular shuttles. The complexity of molecular MTs is an important metric to determine the viability of their application as a subset of molecular mechanisms. One of the major problems in managing the stiffness of MTs is that the previous methods are related to the stability of MTs and cannot be reversed. The development of a way to control the rigidity of MTs in a flexible manner will allow for the robust regulation of MT properties and functions, and there is significant growth in molecular mechanics, molecular robotics, and related fields.

Kabir and his colleagues used trimethylamine N-oxide (TMAO), a molecule that acts as an osmolyte in deep-sea organisms, to study its effect on MTs in space. in vitro gliding assay. TMAO is known to bind proteins under extreme conditions or denaturing of heat, pressure, and chemicals. The TMAO team reported that the complexity of MTs is independent of the effectiveness of the changes in MT buildings.

Deep osmolyte has applications in molecular machinery

Reversible regulation of the rigidity of microtubules (MTs) using trimethylamine N-oxide (TMAO) in the presence of kinesins (Arif Md. Rashedul Kabir, et al. ACS Omega, Jan. 24, 2022). Edited by: Arif Md. Rashedul Kabir, et al. ACS Omega, January 24, 2022

At low TMAO levels (0 mM to 200 mM), the MTs remained upright and rigid and the movement of the MTs was not affected during the test. As the TMAO concentration increased, MTs were shown to bend or pound, and their speed decreased. The team calculated this effect of TMAO on the conformation of MT, indicating that the longevity, a measure of rigidity, MTs were 285 ± 47 μm in the absence of TMAO and decreased to 37 ± 4. μm before 1500 mM TMAO.

The team further reported that the process could be reversed, with the return of the initial duration and speed of MTs once the TMAO was discontinued. These results confirmed that TMAO can be used to further modify the mechanical properties and strength functions of MTs.

Deep osmolyte has applications in molecular machinery

Changing the duration of MTs, by in vitro gliding assay, by increasing the concentration of TMAO from 0 mM to 1500 mM (Arif Md. Rashedul Kabir, et al. ACS Omega, Jan. 24, 2022). Edited by: Arif Md. Rashedul Kabir, et al. ACS Omega, January 24, 2022

Finally, the team investigated how TMAO could change the complexity of MTs. Based on their research, Drs. Arif Md. Rashedul Kabir and his members of the TMAO team decided to apply the turbulence to the difference in energy used by the kinesins in MTs in the experiment; The different force exerted by the kinesins is responsible for changing the stability or duration of persistence of the kinesin propelled MTs.

“This study demonstrated a simple way to measure MT rigidity in an in vitro gliding assay without relying on changes in MT structures,” says Kabir. Future work will focus on explaining how exactly TMAO works, and how to use TMAO to manage the assets and operations of MTs and devices. kinesins, and are essential for molecular machines and molecular robotics.

Deep osmolytes form biomolecular structures with heat

More information:
Arif Md. Rashedul Kabir et al, Controlling the Rigidity of Kinesin-Propelled Microtubules in an In Vitro Gliding Assay Using Deep-Sea Osmolyte Trimethylamine N-Oxide, ACS Omega (2022). DOI: 10.1021 / acsomega.1c06699

Presented by Hokkaido University

Directions: Deep osmolyte applications in molecular machines (2022, April 7) Retrieved 7 April 2022 from molecular-machines.html

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