A new technology to unlock gene regulation

Learning from a single phone: A new technology to unlock gene regulation

Zebrafish notochord nuclei at the 15-somite level. Silver: nuclear DNA (DAPI). color: histone H3K9me3. Found: Phong Nguyen, Franka Rang & Kim de Luca.

How does DNA synthesis regulate the function of genes? To answer this question, a technology that measures gene expression and DNA testing was developed simultaneously by Franka Rang and Kim de Luca, researchers from the Jop Kind team (co -founder at Hubrecht Institute and Oncode Investigator). This technique, called EpiDamID, determines the location of the modified proteins around the bound DNA. It is important to gather information about these mutations because they alter the ability to obtain DNA, and then relate to gene production. Therefore, EpiDamID is valuable for researching early biological development. The results of the study are published in Molecular Phone.

In order for DNA to be incorporated into the nucleus of a cell, it is wrapped around nuclear proteins called histones. Because of the stability of this complex, (i) DNA can be found in other proteins. To determine if the process of gene expression, the translation of DNA into RNA and into proteins, can be done.

DNA testing regulates gene production

The stability of the DNA that surrounds histones is regulated by the addition of molecular groups, called post-translational modifications (PTMs), to the histones. For example, when certain molecules are attached to histones, the DNA strand is released. This makes the DNA easier for certain proteins and stimulates the genes in this part of the DNA to become active, or expressive. In addition, proteins may be important for demonstrating how to accurately recognize and bind to CPUs. This includes transcription: the process of DNA copying.

Regulation of expression, for example through PTMs, is also known as epigenetic regulation. Because the DNA of all cells in the body is the same, it is necessary to modify gene expression in order to (de) activate specific functions in each cell. . For example, heart muscle works differently than skin and therefore requires different genes to be identified.

Monitoring the same systems with EpiDamID

To understand the relevance of PTMs to the presentation, early authors Franka Rang and Kim de Luca developed a new way to determine where the changes are. Using this method, called EpiDamID, the researchers were able to record single samples, although the first methods were able to measure a large group of cells. Knowledge in such a small way would give more information about the difference in DNA volume in each cell, rather than knowledge about the average DNA volume of many cells.

EpiDamID is based on DamID, a technology used to determine the binding of certain DNA -binding proteins. Using EpiDamID, the presence of specific PTMs on histone proteins in single cells can be detected. Compared to other methods, one of the main advantages of this technology is that it requires very little research. In addition, EpiDamID can be used with other techniques, such as microscopy, to study the regulation of gene expression at different levels.

Future ideas

Following the development of this technology, the Kind team will focus on the role of CPUs from the perspective of developmental biology. Because the same systems are being analyzed with EpiDamID, a number of factors are needed to generate the data. This allows researchers to study the early development of an organism from its original cell divisions, when the embryo has only a small number of them.


A study of ‘grammar’ was found after the correction of the person


More information:
Jop Kind, Transcriptome and histone conversion with EpiDamID, Molecular Phone (2022). DOI: 10.1016 / j.molcel.2022.03.009. www.cell.com/molecular-cell/fu fu 1097-2765 (22) 00218-0

Presented by Hubrecht Institute

Directions: Learning from a single phone: New technology to unlock gene regulation (2022, April 1) Retrieved April 1, 2022 from https://phys.org/news/2022-04-cell- technique-unravel-gene.html

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